Which of the following corresponds to the coding strand of the following mRNA?
5’-GUAACGUCA- 3’
A . 5’-GTAACGTCA-3’
B . 5’-CATTGCAGT-3’
C . 5’-ACUGCAAUG- 3’
D . 5’-TGACGTTAC- 3’
Answer: A
Explanation:
The coding strand is NOT the complementary strand; it is the strand of DNA which is complementary to the strand from which mRNA is made. The coding strand will have the same sequence of bases as the produced RNA transcript except with thymine instead of uracil. A useful mnemonic for remembering the nucleotide bases in DNA and RNA is “GCAT PuPy” and “GCAU PuPy.” GCAT/U stand for guanine, cytosine, adenine, thymine, and uracil, and “Pu” and “Py” represent purine and pyrimidine, respectively.
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