Gram-positive bacteria retain the primary crystal violet stain that is applied in the Gram stain because of the cross-linking of the thick peptidoglycan layer in their cell walls. Their cell walls contain less lipids than that of a gram-negative bacteria, and this reduces the permeability of their cell wall to the organic solvents that are used as a decolorizer. When the counterstain is added, it may enter the gram-positive cell, but does not change the color
of the cells.
A thick peptidoglycan is MOST often associated with which of the following types of bacteria?
A . Gram-negative bacteria
B. Gram-positive bacteria
C. Both gram-negative and gram-positive bacteria
Answer: B
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